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- KD/KO Validated
CXCR3B-specific Monoclonal antibody
CXCR3B-specific Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1B2D6
Cat no : 60065-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MOLT-4 cells, HT-29 cells, Jurkat cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 5 publications below |
| FC | See 3 publications below |
Product Information
60065-1-Ig targets CXCR3B-specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | chemokine (C-X-C motif) receptor 3 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | AF469635 |
| Gene Symbol | CXCR3 |
| Gene ID (NCBI) | 2833 |
| RRID | AB_2292376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR3B-specific antibody 60065-1-Ig | Download protocol |
| IHC protocol for CXCR3B-specific antibody 60065-1-Ig | Download protocol |
| IF protocol for CXCR3B-specific antibody 60065-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol Pericyte regulation of vascular remodeling through the CXC receptor 3.
| ||
Int J Obes (Lond) Upregulation of angiostatic chemokines IP-10/CXCL10 and I-TAC/CXCL11 in human obesity and their implication for adipose tissue angiogenesis. | ||
Mol Cancer Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion. | ||
Cell Commun Signal Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer. | ||
J Biol Chem A novel CXCR3-B chemokine receptor-induced growth-inhibitory signal in cancer cells is mediated through the regulation of Bach-1 protein and Nrf2 protein nuclear translocation.
| ||
Food Funct Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway. |
