CoraLite® Plus 488 Anti-Mouse CXCL1 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL488-98047

  1x10^6 untreated or LPS and Brefeldin A treated mouse splenocytes were intracellularly stained with APC Anti-Mouse TNFα and 0.125 ug CoraLite® Plus 488 Anti-Mouse CXCL1 Rabbit Recombinant Antibody (CL488-98047, Clone: 240268D2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CXCL1 Recombinant Antibody for FC (Intra)

Cat No. CL488-98047

Clone No.240268D2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Growth-regulated alpha protein, chemokine (C X C motif) ligand 1, 240268D2

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98047 targets CXCL1 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-X-C motif) ligand 1
• Calculated Molecular Weight: 10kd
• GenBank Accession Number: NM_008176
• Gene Symbol: Cxcl1
• Gene ID (NCBI): 14825
• RRID: AB_3673389
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12850-1
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.