Anti-Mouse CXCL1 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98047-1-RR

  1x10^6 untreated or LPS and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.125 ug Anti-Mouse CXCL1 Rabbit Recombinant Antibody. Cells were then stained with APC Anti-Mouse TNFα. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98047-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98047-1-RR against Mouse CXCL1 were performed. The affinity constant is 35.4 nM.

CXCL1 {ptg:Brand} Recombinant Antibody for FC (Intra)

Cat No. 98047-1-RR

Clone No.240268D2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Growth-regulated alpha protein, chemokine (C X C motif) ligand 1, 240268D2

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98047-1-RR targets CXCL1 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-X-C motif) ligand 1
• Calculated Molecular Weight: 10kd
• GenBank Accession Number: NM_008176
• Gene Symbol: Cxcl1
• Gene ID (NCBI): 14825
• RRID: AB_3672194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12850-1
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.