CTPS2 Monoclonal antibody, PBS Only (Capture)

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3B8

Cat no : 67734-1-PBS

Synonyms

UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II, CTP synthase 2

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Validation Data Gallery

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  WB analysis using 67734-1-Ig (same clone as 67734-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67734-1-Ig (same clone as 67734-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50097-1

  Sandwich ELISA standard curve of MP50097-1, CTPS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67734-1-PBS. Detection antibody: 67734-2-PBS. Standard:Ag30258. Range: 0.195-50 ng/mL.

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  IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 67734-1-Ig (same clone as 67734-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

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Product Information

67734-1-PBS targets CTPS2 as part of a matched antibody pair:

MP50097-1: 67734-1-PBS capture and 67734-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CTPS2 fusion protein Ag30258
• Full Name: CTP synthase II
• Calculated Molecular Weight: 586 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034986
• Gene Symbol: CTPS2
• Gene ID (NCBI): 56474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.