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CTPS2 Monoclonal antibody

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B8

Cat no : 67734-1-Ig

Synonyms

UTP ammonia ligase 2, EC:6.3.4.2, CTP synthetase 2, CTP synthase II, CTP synthase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTPS2 Monoclonal antibody (67734-1-Ig)

    WB analysis using 67734-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of U2OS cells using CTPS2 Monoclonal antibody (67734-1-Ig)

    WB analysis of U2OS using 67734-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IHC staining of human prostate cancer using 67734-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IHC staining of human prostate cancer using 67734-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CTPS2 Monoclonal antibody (67734-1-Ig)

    IF Staining of human prostate cancer using 67734-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CTPS2 Monoclonal antibody (67734-1-Ig)

    IF Staining of PC-12 using 67734-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67734-1-Ig targets CTPS2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CTPS2 fusion protein Ag30258
Full Name CTP synthase II
Calculated Molecular Weight 586 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC034986
Gene Symbol CTPS2
Gene ID (NCBI) 56474
RRIDAB_2882918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Protocols

Product Specific Protocols
WB protocol for CTPS2 antibody 67734-1-IgDownload protocol
IHC protocol for CTPS2 antibody 67734-1-IgDownload protocol
IF protocol for CTPS2 antibody 67734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols