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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11931

Synonyms

CSPP, CSPP1, FLJ22490, FLJ38886

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CSPP1 Polyclonal antibody (CL594-11931)

    IF Staining of HeLa using CL594-11931

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CSPP1 antibody (CL594-11931) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11931 targets CSPP1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSPP1 fusion protein Ag2521
Full Name centrosome and spindle pole associated protein 1
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 141 kDa
GenBank Accession NumberBC029445
Gene Symbol CSPP1
Gene ID (NCBI) 79848
RRIDAB_2919776
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Protocols

Product Specific Protocols
IF protocol for CL594 CSPP1 antibody CL594-11931Download protocol
Standard Protocols
Click here to view our Standard Protocols