CoraLite®594-conjugated CSPP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSPP1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11931

Synonyms

CSPP, CSPP1, FLJ22490, FLJ38886

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Validation Data Gallery

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  IF Staining of HeLa using CL594-11931

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CSPP1 antibody (CL594-11931) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11931 targets CSPP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSPP1 fusion protein Ag2521
• Full Name: centrosome and spindle pole associated protein 1
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: BC029445
• Gene Symbol: CSPP1
• Gene ID (NCBI): 79848
• RRID: AB_2919776
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo