CoraLite® Plus 488-conjugated CRMP5 Monoclonal antibody

CRMP5 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F2D8

Cat no : CL488-68272

Synonyms

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL488-68272

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 CRMP5 antibody (CL488-68272, Clone: 3F2D8 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68272 targets CRMP5 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRMP5 fusion protein Ag0783
• Full Name: dihydropyrimidinase-like 5
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC002874
• Gene Symbol: CRMP5
• Gene ID (NCBI): 56896
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Collapsin response mediator proteins (CRMPs) are a family consisting of five homologous cytosolic phosphoproteins (CRMP1-5). All five CRMPs are abundantly expressed in the developing brain and the adult nervous system. CRMP5 is phylogenetically divergent from the other four CRMPs, sharing only 50% homology; this protein plays an important role in modulating filopodial dynamics and, in turn, restricting neuronal growth cone development.