CREB1 Monoclonal antibody, PBS Only

CREB1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11C1

Cat no : 67927-1-PBS

Synonyms

CREB, CREB 1, CREB1

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Validation Data Gallery

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  WB analysis using 67927-1-Ig (same clone as 67927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67927-1-Ig (CREB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CREB1 antibody (67927-1-Ig, Clone: 1E11C1 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67927-1-PBS targets CREB1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CREB1 fusion protein Ag2852
• Full Name: cAMP responsive element binding protein 1
• Calculated Molecular Weight: 341 aa, 35 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC010636
• Gene Symbol: CREB1
• Gene ID (NCBI): 1385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).