CREB1 Monoclonal antibody

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• KD/KO Validated

CREB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1E11C1

Cat no : 67927-1-Ig

Synonyms

CREB, CREB 1, CREB1

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Validation Data Gallery

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  WB analysis using 67927-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67927-1-Ig (CREB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 67927-1-Ig

  WB result of CREB1 antibody (67927-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells.

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  IHC staining of human prostate cancer using 67927-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67927-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67927-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67927-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67927-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CREB1 antibody (67927-1-Ig, Clone: 1E11C1 ) at dilution of 1:8000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human prostate cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67927-1-Ig targets CREB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CREB1 fusion protein Ag2852
• Full Name: cAMP responsive element binding protein 1
• Calculated Molecular Weight: 341 aa, 35 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC010636
• Gene Symbol: CREB1
• Gene ID (NCBI): 1385
• RRID: AB_2918679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury; Authors - Wenyu Shi
mouse	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun
human	WB	BMC Cancer; Identification of TLN1 as a prognostic biomarker to effect cell proliferation and differentiation in acute myeloid leukemia; Authors - Di Cui