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CPT2 Recombinant antibody, PBS Only

CPT2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242381C5

Cat no : 85013-4-PBS

Synonyms

EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPT2 Recombinant antibody (85013-4-RR)

    WB analysis using 85013-4-RR (same clone as 85013-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85013-4-RR (CPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85013-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85013-4-RR

    Affinity and Kinetic Characterization of 85013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85013-4-RR against Human CPT2 were performed. The affinity constant is 0.261 nM.

Product Information

85013-4-PBS targets CPT2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPT2 fusion protein Ag24897
Full Name carnitine palmitoyltransferase 2
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC002445
Gene Symbol CPT2
Gene ID (NCBI) 1376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).