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  • Western Blot (WB) analysis of various lysates using CPT2 Recombinant antibody (85013-4-RR)

    WB analysis using 85013-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85013-4-RR (CPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85013-4-RR

    Affinity and Kinetic Characterization of 85013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85013-4-RR against Human CPT2 were performed. The affinity constant is 0.261 nM.

CPT2 Recombinant antibody

CPT2 Recombinant Antibody for WB, ELISA
Cat No. 85013-4-RR
Clone No.242381C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells, T-47D cells, LNCaP cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85013-4-RR targets CPT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPT2 fusion protein Ag24897 Predict reactive species
Full Name carnitine palmitoyltransferase 2
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC002445
Gene Symbol CPT2
Gene ID (NCBI) 1376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

Protocols

Product Specific Protocols
WB protocol for CPT2 antibody 85013-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols