CPT2 Recombinant antibody

CPT2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242381C5

Cat no : 85013-4-RR

Synonyms

EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial

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Validation Data Gallery

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  WB analysis using 85013-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85013-4-RR (CPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85013-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85013-4-RR against Human CPT2 were performed. The affinity constant is 0.261 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HepG2 cells, T-47D cells, LNCaP cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85013-4-RR targets CPT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CPT2 fusion protein Ag24897
• Full Name: carnitine palmitoyltransferase 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002445
• Gene Symbol: CPT2
• Gene ID (NCBI): 1376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).