CoraLite® Plus 488-conjugated CPT1B-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

CPT1B-specific Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22170

Synonyms

CPT I, CPT1 M, CPT1B, CPTI M, FLJ55729, FLJ58750, KIAA1670, M CPT1, MCCPT1

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-22170

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 CPT1B-specific antibody (CL488-22170) at dilution of 1:200.

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  IF Staining of mouse heart using CL488-22170

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 CPT1B-specific antibody (CL488-22170) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22170 targets CPT1B-specific in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT1B-specific fusion protein Ag17840
• Full Name: carnitine palmitoyltransferase 1B (muscle)
• Calculated Molecular Weight: 567 aa, 64 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC131570
• Gene Symbol: CPT1B
• Gene ID (NCBI): 1375
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.