Tested Applications
Positive IF-P detected in | mouse heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-22170 targets CPT1B-specific in IF-P applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CPT1B-specific fusion protein Ag17840 Predict reactive species |
Full Name | carnitine palmitoyltransferase 1B (muscle) |
Calculated Molecular Weight | 567 aa, 64 kDa |
Observed Molecular Weight | 75-85 kDa |
GenBank Accession Number | BC131570 |
Gene Symbol | CPT1B |
Gene ID (NCBI) | 1375 |
RRID | AB_2923748 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92523 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 CPT1B-specific antibody CL488-22170 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |