CoraLite® Plus 488-conjugated CPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CPS1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18703

Synonyms

CPS1, CPSase I

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-18703

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CPS1 antibody (CL488-18703) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18703 targets CPS1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: carbamoyl-phosphate synthetase 1, mitochondrial
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 140-165 kDa
• GenBank Accession Number: BC140943
• Gene Symbol: CPS1
• Gene ID (NCBI): 1373
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.