CoraLite®594-conjugated COXIV Polyclonal antibody

• Featured Product
• KD/KO Validated

COXIV Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11242

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-11242

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 COXIV antibody (CL594-11242) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11242 targets COXIV in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COXIV fusion protein Ag1640
• Full Name: cytochrome c oxidase subunit IV isoform 1
• Calculated Molecular Weight: 19.6 kDa
• Observed Molecular Weight: 17-18 kDa
• GenBank Accession Number: BC021236
• Gene Symbol: COX IV
• Gene ID (NCBI): 1327
• RRID: AB_2919759
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.