CoraLite® Plus 488-conjugated COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

4A3D9

Cat no : CL488-60237

Synonyms

CLP, Coactosin like protein, COTL1, FLJ43657

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-60237

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated COTL1 antibody (CL488-60237, Clone: 4A3D9 ) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-60237

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 COTL1 antibody (CL488-60237, Clone: 4A3D9 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of CL488-60237 is COTL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COTL1 fusion protein Ag1230
• Full Name: coactosin-like 1 (Dictyostelium)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC010884
• Gene Symbol: COTL1
• Gene ID (NCBI): 23406
• RRID: AB_2883124
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Publications

Species	Application	Title
human	IF	Front Cell Dev Biol; Coactosin-like protein 1 regulates integrity and repair of model intestinal epithelial barriers via actin binding dependent and independent mechanisms; Authors - Susana Lechuga