CNNM4 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HEK-293 using 83824-4-RR (same clone as 83824-4-PBS)

  WB result of CNNM4 antibody (83824-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNNM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 83824-4-PBS in a different storage buffer formulation.

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  WB analysis using 83824-4-RR (same clone as 83824-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83824-4-RR (CNNM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83824-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00802-3

  Cytometric bead array standard curve of MP00802-3, CNNM4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83824-4-PBS. Detection antibody: 83824-2-PBS. Standard: Ag5121. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83824-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83824-4-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

CNNM4 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 83824-4-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Product Information

83824-4-PBS targets CNNM4 as part of a matched antibody pair:

MP00802-3: 83824-4-PBS capture and 83824-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CNNM4 fusion protein Ag5121
• Full Name: cyclin M4
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC063295
• Gene Symbol: CNNM4
• Gene ID (NCBI): 26504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P4Q7
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).