• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CNNM4 Recombinant antibody

CNNM4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240873E9

Cat no : 83824-4-RR

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CNNM4 Recombinant antibody (83824-4-RR)

    WB analysis using 83824-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83824-4-RR (CNNM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83824-4-RR

    Affinity and Kinetic Characterization of 83824-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83824-4-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83824-4-RR is CNNM4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CNNM4 fusion protein Ag5121
Full Name cyclin M4
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

Protocols

Product Specific Protocols
WB protocol for CNNM4 antibody 83824-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols