CLEC14A Recombinant antibody, PBS Only

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  WB analysis using 84327-1-RR (same clone as 84327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84327-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

CLEC14A {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA

Cat No. 84327-1-PBS

Clone No.241206D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

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Product Information

84327-1-PBS targets CLEC14A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLEC14A fusion protein Ag21081
• Full Name: C-type lectin domain family 14, member A
• Calculated Molecular Weight: 490 aa, 52 kDa
• Observed Molecular Weight: 52 kDa, 100 kDa
• GenBank Accession Number: BC031567
• Gene Symbol: CLEC14A
• Gene ID (NCBI): 161198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86T13
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).