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  • Western Blot (WB) analysis of various lysates using CLEC14A Recombinant antibody (84327-1-RR)

    WB analysis using 84327-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84327-1-RR

    Affinity and Kinetic Characterization of 84327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

CLEC14A Recombinant antibody

CLEC14A {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 84327-1-RR
Clone No.241206D3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84327-1-RR targets CLEC14A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLEC14A fusion protein Ag21081 Predict reactive species
Full Name C-type lectin domain family 14, member A
Calculated Molecular Weight 490 aa, 52 kDa
Observed Molecular Weight 52 kDa, 100 kDa
GenBank Accession NumberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
RRIDAB_3671868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

Protocols

Product Specific Protocols
WB protocol for CLEC14A antibody 84327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols