CLEC14A Recombinant antibody

CLEC14A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241206D3

Cat no : 84327-1-RR

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

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Validation Data Gallery

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  WB analysis using 84327-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84327-1-RR is CLEC14A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLEC14A fusion protein Ag21081
• Full Name: C-type lectin domain family 14, member A
• Calculated Molecular Weight: 490 aa, 52 kDa
• Observed Molecular Weight: 52 kDa, 100 kDa
• GenBank Accession Number: BC031567
• Gene Symbol: CLEC14A
• Gene ID (NCBI): 161198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).