CoraLite® Plus 647 Anti-Human CLEC10A/CD301 Rabbit Recombinant Antibody

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  FC experiment of Human immature monocyte-derived dendritic cells using CL647-98245

  1x10^6 FC receptor blocked Human immature monocyte-derived dendritic cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CLEC10A/CD301 Rabbit RecAb (CL647-98245, Clone: 241857C11) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

CLEC10A/CD301 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98245

Clone No.241857C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CLEC10A, 241857C11, CD301, CLECSF13, CLECSF14

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Tested Applications

• Positive FC detected in: Human immature monocyte-derived dendritic cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98245 targets CLEC10A/CD301 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLEC10A/CD301 fusion protein Eg1892
• Full Name: C-type lectin domain family 10, member A
• Calculated Molecular Weight: 316 aa, 35 kDa
• GenBank Accession Number: BC039011
• Gene Symbol: CLEC10A
• Gene ID (NCBI): 10462
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IUN9
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).