CISD2 Recombinant antibody, PBS Only

CISD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6H13

Cat no : 82802-10-PBS

Synonyms

Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2, CDGSH iron sulfur domain 2

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Validation Data Gallery

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  WB analysis using 82802-10-RR (same clone as 82802-10-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82802-10-RR (CISD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82802-10-RR (same clone as 82802-10-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (82802-10-RR, Clone: 6H13 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82802-10-RR (same clone as 82802-10-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CISD2 Recombinant antibody (82802-10-RR, Clone:6H13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82802-10-RR

  Biolayer interferometry (BLl) kinetic assays of 82802-10-RR against Human CISD2 were performed. The affinity constant is 3.07 nM.

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Product Information

82802-10-PBS targets CISD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CISD2 fusion protein Ag4172
• Full Name: CDGSH iron sulfur domain 2
• Calculated Molecular Weight: 135 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC032300
• Gene Symbol: CISD2
• Gene ID (NCBI): 493856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.