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CISD2 Recombinant antibody

CISD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6H13

Cat no : 82802-10-RR

Synonyms

Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2, CDGSH iron sulfur domain 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CISD2 Recombinant antibody (82802-10-RR)

    WB analysis using 82802-10-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82802-10-RR (CISD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CISD2 Recombinant antibody (82802-10-RR)

    IF Staining of HepG2 using 82802-10-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (82802-10-RR, Clone: 6H13 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CISD2 Recombinant antibody (82802-10-RR)

    FC experiment of HepG2 using 82802-10-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CISD2 Recombinant antibody (82802-10-RR, Clone:6H13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82802-10-RR

    Affinity and Kinetic Characterization of 82802-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82802-10-RR against Human CISD2 were performed. The affinity constant is 3.07 nM.

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, L02 cells, K-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82802-10-RR targets CISD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CISD2 fusion protein Ag4172
Full Name CDGSH iron sulfur domain 2
Calculated Molecular Weight 135 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC032300
Gene Symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for CISD2 antibody 82802-10-RRDownload protocol
IHC protocol for CISD2 antibody 82802-10-RRDownload protocol
IF protocol for CISD2 antibody 82802-10-RRDownload protocol
FC protocol for CISD2 antibody 82802-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols