CoraLite® Plus 750-conjugated CHMP4B Recombinant antibody

CHMP4B Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2N22

Cat no : CL750-81691-2

Synonyms

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

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Validation Data Gallery

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  WB analysis using CL750-81691-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81691-2 (CHMP4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using CL750-81691-2

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated CHMP4B Recombinant antibody (CL750-81691-2, Clone:2N22)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, HuH-7 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-81691-2 is CHMP4B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHMP4B fusion protein Ag4544
• Full Name: chromatin modifying protein 4B
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC033859
• Gene Symbol: CHMP4B
• Gene ID (NCBI): 128866
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).