CoraLite® Plus 488-conjugated CHI3L1/YKL40 Recombinant antibody

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  IF Staining of THP-1 using CL488-83685-4

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 CHI3L1/YKL40 antibody (CL488-83685-4, Clone: 240691F11 ) at dilution of 1:200.

CHI3L1/YKL40 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-83685-4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

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Tested Applications

• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83685-4 targets CHI3L1/YKL40 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHI3L1/YKL40 fusion protein Ag33123
• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC008568
• Gene Symbol: CHI3L1
• Gene ID (NCBI): 1116
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36222
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)