View 3D epitope

CHI3L1/YKL40 Recombinant antibody

CHI3L1/YKL40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83685-4-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

--/ -

Freight/Packing: --

Quantity
Select a country first to see the price

Tested Applications

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83685-4-RR targets CHI3L1/YKL40 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHI3L1/YKL40 fusion protein Ag33123 Predict reactive species
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight40-43 kDa
GenBank Accession NumberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
RRIDAB_3671287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Protocols

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
IF protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
FC protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...