CHI3L1/YKL40 Recombinant antibody, PBS Only

• 

  WB analysis of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 83685-4-RR (CHI3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1 antibody (83685-4-RR, Clone: 240691F11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

• 

  FC experiment of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug CHI3L1 Recombinant antibody (83685-4-RR, Clone:240691F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83685-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83685-4-RR against Human CHI3L1 were performed. The affinity constant is 3.14 nM.

CHI3L1/YKL40 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83685-4-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39

---

Product Information

83685-4-PBS targets CHI3L1/YKL40 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CHI3L1/YKL40 fusion protein Ag33123
• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC008568
• Gene Symbol: CHI3L1
• Gene ID (NCBI): 1116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P36222
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)