CoraLite® Plus 488-conjugated CEP63 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP63 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16268

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

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Validation Data Gallery

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  IF Staining of MDCK using CL488-16268

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 CEP63 antibody (CL488-16268) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP63 fusion protein Ag9335
• Full Name: centrosomal protein 63kDa
• Calculated Molecular Weight: 703 aa, 81 kDa
• Observed Molecular Weight: 57-75 kDa
• GenBank Accession Number: BC014050
• Gene Symbol: CEP63
• Gene ID (NCBI): 80254
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).