CEP63 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP63 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 16268-1-AP

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63

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Validation Data Gallery

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  WB analysis of HEK-293 using 16268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16268-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16268-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16268-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16268-1-AP (CEP63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16268-1-AP

  IP result of anti-CEP63 (IP:16268-1-AP, 3ug; Detection:16268-1-AP 1:500) with HEK-293 cells lysate 1700ug.

• 

  IF Staining of MDCK using 16268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP63 antibody (16268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, HeLa cells, mouse thymus tissue
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 9 publications below
• IP: See 3 publications below

Product Information

16268-1-AP targets CEP63 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP63 fusion protein Ag9335
• Full Name: centrosomal protein 63kDa
• Calculated Molecular Weight: 703 aa, 81 kDa
• Observed Molecular Weight: 57-75 kDa
• GenBank Accession Number: BC014050
• Gene Symbol: CEP63
• Gene ID (NCBI): 80254
• RRID: AB_2077079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

Publications

Species	Application	Title
mouse		Nat Cell Biol; Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.; Authors - Olivier Mercey
mouse	WB,IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
human	WB, IF, IP	Dev Cell; A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.; Authors - Zhen Zhao ; KO Validated
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
human	IF	Nat Commun; Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.; Authors - Søs Grønbæk Holdgaard
mouse	IF	Nat Commun; Autophagy controls centrosome number by degrading Cep63.; Authors - Yuichiro Watanabe