CoraLite® Plus 488-conjugated CEP170/CEP170L Polyclonal antibody

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  IF Staining of HeLa using CL488-18899

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CEP170/CEP170L antibody (CL488-18899) at dilution of 1:200.

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  FC experiment of HeLa using CL488-18899

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CEP170/CEP170L Polyclonal antibody (CL488-18899)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

CEP170/CEP170L Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-18899

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

KIAA0470, CEP170, CEP170L, KIAA0470L

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18899 targets CEP170/CEP170L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP170/CEP170L fusion protein Ag13532
• Full Name: centrosomal protein 170kDa-like
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 170-200 kDa
• GenBank Accession Number: BC014590
• Gene Symbol: CEP170
• Gene ID (NCBI): 645455
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CEP170, also named as FAM68A, KAB and KIAA0470, is a fork head-associated (FHA) domain protein. CEP170L is a part of CEP170. The gene CEP170 has some isoforms with MW 170-200 kDa, 160 kDa and some fragments 30-40 kDa, ~75 kDa.