CoraLite® Plus 488-conjugated CENPJ Polyclonal antibody

• Featured Product
• KD/KO Validated

CENPJ Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11517

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAG 3 associated protein, LAP, LIP1, LYST interacting protein 1, MCPH6

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Validation Data Gallery

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  IF Staining of HeLa using CL488-11517

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CENPJ antibody (CL488-11517) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11517 targets CENPJ in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPJ fusion protein Ag2078
• Full Name: centromere protein J
• Calculated Molecular Weight: 1338 aa, 153 kDa
• GenBank Accession Number: BC024209
• Gene Symbol: CENPJ
• Gene ID (NCBI): 55835
• RRID: AB_2919030
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.