CDK8 Recombinant antibody, PBS Only

CDK8 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240598G9

Cat no : 83621-6-PBS

Synonyms

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

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Validation Data Gallery

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  WB analysis using 83621-6-RR (same clone as 83621-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83621-6-RR (same clone as 83621-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83621-6-RR (same clone as 83621-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 83621-6-RR (same clone as 83621-6-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83621-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

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Product Information

The immunogen of 83621-6-PBS is CDK8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDK8 fusion protein Ag17148
• Full Name: cyclin-dependent kinase 8
• Calculated Molecular Weight: 464 aa, 53 kDa
• GenBank Accession Number: BC069634
• Gene Symbol: CDK8
• Gene ID (NCBI): 1024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.