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CDK8 Recombinant antibody

CDK8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240598G9

Cat no : 83621-6-RR

Synonyms

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK8 Recombinant antibody (83621-6-RR)

    WB analysis using 83621-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK8 Recombinant antibody (83621-6-RR)

    IF Staining of HepG2 using 83621-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK8 Recombinant antibody (83621-6-RR)

    IF Staining of HepG2 using 83621-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Caco-2 cells using CDK8 Recombinant antibody (83621-6-RR)

    FC experiment of Caco-2 using 83621-6-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83621-6-RR

    Affinity and Kinetic Characterization of 83621-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HCT 116 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83621-6-RR is CDK8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK8 fusion protein Ag17148
Full Name cyclin-dependent kinase 8
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC069634
Gene Symbol CDK8
Gene ID (NCBI) 1024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDK8 antibody 83621-6-RRDownload protocol
IF protocol for CDK8 antibody 83621-6-RRDownload protocol
FC protocol for CDK8 antibody 83621-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols