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CoraLite® Plus 488-conjugated CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55103

Synonyms

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CDK3 Polyclonal anti (CL488-55103)

    FC experiment of HeLa using CL488-55103

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDK3 (CL488-55103) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55103 targets CDK3 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin-dependent kinase 3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_001258
Gene Symbol CDK3
Gene ID (NCBI) 1018
RRIDAB_2919220
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CDK3 antibody CL488-55103Download protocol
Standard Protocols
Click here to view our Standard Protocols