CDC37 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CDC37 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10218-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10218-1-AP (same clone as 10218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 10218-1-AP (same clone as 10218-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10218-1-AP (same clone as 10218-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10218-1-AP (same clone as 10218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of HeLa cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using CDC37 antibody 10218-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of A431 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10218-1-AP (same clone as 10218-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 10218-1-AP (same clone as 10218-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDC37 Polyclonal antibody (10218-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10218-1-PBS in a different storage buffer formulation.

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Product Information

10218-1-PBS targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC37 fusion protein Ag0267
• Full Name: cell division cycle 37 homolog (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC000083
• Gene Symbol: CDC37
• Gene ID (NCBI): 11140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.