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  • Featured Product
  • KD/KO Validated

CDC37 Polyclonal antibody

CDC37 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10218-1-AP

Synonyms

p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, Hsp90 co-chaperone Cdc37, Hsp90 co chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using CDC37 Polyclonal antibody (10218-1-AP)

    WB analysis of HEK-293 using 10218-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CDC37 Polyclonal antibody (10218-1-AP)

    WB analysis of A431 using 10218-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CDC37 Polyclonal antibody (10218-1-AP)

    WB analysis of Jurkat using 10218-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDC37 Polyclonal antibody (10218-1-AP)

    WB analysis of HEK-293 using 10218-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using CDC37 Polyclonal antibody (10218-1-AP)

    WB analysis of COS-7 using 10218-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CDC37 Polyclonal antibody (10218-1-AP)

    IP experiment of HEK-293 using 10218-1-AP

    IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC37 Polyclonal antibody (10218-1-AP)

    IHC staining of human colon cancer using 10218-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC37 Polyclonal antibody (10218-1-AP)

    IHC staining of human colon cancer using 10218-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10218-1-AP (CDC37 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC37 Polyclonal antibody (10218-1-AP)

    IF Staining of HeLa using 10218-1-AP

    Immunofluorescent analysis of HeLa cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CDC37 Polyclonal antibody (10218-1-AP)

    IF Staining of A431 using 10218-1-AP

    Immunofluorescent analysis of A431 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CDC37 Polyclonal antibody (10218-1-AP)

    IF Staining of MCF-7 using 10218-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CDC37 antibody 10218-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDC37 Polyclonal antibody (10218-1-AP)

    IF Staining of HEK-293 using 10218-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10218-1-AP (Cdc37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using CDC37 Polyclonal antibody (10218-1-AP)

    FC experiment of MCF-7 using 10218-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDC37 Polyclonal antibody (10218-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, COS-7 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A431 cells, HEK-293 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10218-1-AP targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC37 fusion protein Ag0267
Full Name cell division cycle 37 homolog (S. cerevisiae)
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC000083
Gene Symbol CDC37
Gene ID (NCBI) 11140
RRIDAB_2077735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Protocols

Product Specific Protocols
WB protocol for CDC37 antibody 10218-1-APDownload protocol
IHC protocol for CDC37 antibody 10218-1-APDownload protocol
IF protocol for CDC37 antibody 10218-1-APDownload protocol
IP protocol for CDC37 antibody 10218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
View Article
humanIHC

Comput Biol Med

Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data

Authors - Zehua Wang
View Article
humanWB

Oncogene

Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4.

Authors - Wenzhi Jia
View Article
humanWB

Int J Mol Sci

(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.

Authors - Chen Chen
View Article
humanWB, IP

Pharmacol Res

Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.

Authors - Dongrong Zhu
View Article
humanWB

Inflammation

Inhibition of Cdc37 Ameliorates Arthritis in Collagen-Induced Arthritis Rats by Inhibiting Synoviocyte Proliferation and Migration Through the ERK Pathway

Authors - Weiwei Sun
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Iram (Verified Customer) (02-11-2022)

Very good antibody for IP and western

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells
FH

Kyosuke (Verified Customer) (06-12-2019)

It works good on HEK293T cells for Western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T