CDC25A Recombinant antibody

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  IHC staining of human stomach cancer using 84145-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84145-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84145-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84145-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CDC25A Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84145-2-RR

Clone No.240813F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

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Tested Applications

• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84145-2-RR targets CDC25A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cell division cycle 25 homolog A (S. pombe)
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: NM_001789
• Gene Symbol: CDC25A
• Gene ID (NCBI): 993
• RRID: AB_3671708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P30304
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.