CDC25A Recombinant antibody, PBS Only

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  IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

CDC25A Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84145-2-PBS

Clone No.240813F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

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Product Information

84145-2-PBS targets CDC25A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cell division cycle 25 homolog A (S. pombe)
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: NM_001789
• Gene Symbol: CDC25A
• Gene ID (NCBI): 993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P30304
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.