FC experiment of human peripheral blood leukocytes using 98172-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone:241704E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone:241704E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98172-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone: 241704E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone: 241704E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
Affinity and Kinetic Characterization of 98172-1-RR
Biolayer interferometry (BLl) kinetic assays of 98172-1-RR
against Human CD94 were performed. The affinity constant is below 3.17 nM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98172-1-RR targets CD94 in FC applications and shows reactivity with human samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
CD94, also known as KLRD1, is a membrane protein that is preferentially expressed on NK cells. CD94 can form disulfide-bonded heterodimers with NKG2 family members. The CD94/NKG2A heterodimer constitutes an inhibitory receptor that recognizes HLA-E.
FC experiment of human peripheral blood leukocytes using 98172-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone:241704E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98172-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone: 241704E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98172-1-RR
Biolayer interferometry (BLl) kinetic assays of 98172-1-RR
against Human CD94 were performed. The affinity constant is below 3.17 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
