Anti-Human CD94 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98172-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone:241704E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98172-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD94 Rabbit Recombinant Antibody (98172-1-RR, Clone: 241704E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98172-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98172-1-RR against Human CD94 were performed. The affinity constant is below 3.17 nM.

CD94 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98172-1-RR

Clone No.241704E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241704E11, Killer cell lectin-like receptor subfamily D member 1, KLRD1, KP43, NK cell receptor

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98172-1-RR targets CD94 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: killer cell lectin-like receptor subfamily D, member 1
• Calculated Molecular Weight: 179 aa, 20 kDa
• GenBank Accession Number: BC028009
• Gene Symbol: CD94
• Gene ID (NCBI): 3824
• RRID: AB_3672315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13241
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD94, also known as the killer cell lectin-like receptor (KLR) family member, is a type II integral membrane protein related to the C-type lectin superfamily. It is specifically expressed on natural killer (NK) cells and a subset of T cells, and plays a crucial role in stimulating NK cell cytolysis and the release of tumor necrosis factor. CD94 can form heterodimers with different NKG2 proteins (e.g., NKG2A, NKG2C, NKG2E, or NKG2F) to balance the function of NK cells and CD8+ T cells.