Cardinal Red™ Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

GHI/75

Cat no : CR-65238

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

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Validation Data Gallery

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  FC experiment of human PBMCs using CR-65238

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD85j / LILRB1 (CR-65238, Clone:GHI/75) (red) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CR-65238

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD85j / LILRB1 (CR-65238, Clone:GHI/75) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65238 targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human Hairy Cell Leukemia Spleen Cells
• Full Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC015731
• Gene Symbol: LILRB1
• Gene ID (NCBI): 10859
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: N/A
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.