Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/75

Cat no : 65238-1-Ig

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

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Validation Data Gallery

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  FC experiment of human PBMCs using 65238-1-Ig

  1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Monocytes are gated. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65238-1-Ig

  1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000, or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10). Monocytes are gated. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65238-1-Ig targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human Hairy Cell Leukemia Spleen Cells
• Full Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC015731
• Gene Symbol: LILRB1
• Gene ID (NCBI): 10859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.