Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody, PBS Only

CD83 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

HB15e

Cat no : 65581-1-PBS

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

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Validation Data Gallery

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  FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR (same clone as 65581-1-PBS)

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65581-1-PBS in a different storage buffer formulation.

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Product Information

65581-1-PBS targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human CD83-transfected Cos cells
• Full Name: CD83 molecule
• Calculated Molecular Weight: 205 aa, 23 kDa
• GenBank Accession Number: BC030830
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).