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Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody

CD83 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

HB15e

Cat no : 65581-1-MR

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human monocyte-derived mature dendritic cells using Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant An (65581-1-MR)

    FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR

    1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected inHuman monocyte-derived mature dendritic cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65581-1-MR targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human CD83-transfected Cos cells
Full Name CD83 molecule
Calculated Molecular Weight 205 aa, 23 kDa
GenBank Accession NumberBC030830
Gene Symbol CD83
Gene ID (NCBI) 9308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

Protocols

Product Specific Protocols
FC protocol for CD83 antibody 65581-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols