PE Anti-Human CD81 (5A6)

CD81 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

5A6

Cat no : PE-65195

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 5A6

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Validation Data Gallery

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  FC experiment of human PBMCs using PE-65195

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD81 (PE-65195, Clone:5A6) or PE Mouse IgG1 Isotype Control (PE-65124). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65195

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD81 (PE-65195, Clone:5A6) or PE Mouse IgG1 Isotype Control (PE-65124). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65195 targets CD81 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human OCI-LY8 cell line
• Full Name: CD81 molecule
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC002978
• Gene Symbol: CD81
• Gene ID (NCBI): 975
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).