Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65195-1-Ig targets CD81 in FC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human OCI-LY8 cell line Predict reactive species |
| Full Name | CD81 molecule |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | BC002978 |
| Gene Symbol | CD81 |
| Gene ID (NCBI) | 975 |
| ENSEMBL Gene ID | ENSG00000110651 |
| RRID | AB_2918465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60033 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD81 antibody 65195-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD81 (5A6) monoclonal antibody (Cat# 65195-1-Ig) has 2 citations, published in ACS Applied Bio Materials and Journal of Virology (both 2025). The research fields encompass extracellular vesicle biology and membrane interfacial interactions, as well as viral exosome-mediated secretion and immune evasion. Cited applications include ELISA and flow cytometry, both validated in human samples.
| Species | Application | Title |
|---|---|---|
ACS Appl Bio Mater Curvature-Sensing Peptide Functions as a Membrane Interfactant That Glues Small Extracellular Vesicles to Cell Membranes and Enhances Vesicle Cellular Uptake. | ||
J Virol Rab27a-mediated exosome secretion facilitates classical swine fever virus release and immune evasion. |
Reviews
What customers say
One reviewer tested this antibody for Western Blot on HEK293T lysates at 1:500 dilution and reported no detection at the expected molecular weight. The reviewer noted the product is not advertised for WB and rated it 3/5, acknowledging it may perform as intended for its validated application (flow cytometry). Overall, the feedback suggests the antibody is suitable for its intended use but should not be expected to work in unvalidated applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (10-02-2024) | I tried this antibody for western blotting (because this clone has been shown to work in WB in publications) but unfortunately it did not detect any protein at the right size in lysates from HEK293T cells (at 1:500). It is not advertised for use in western blotting and indeed it did not work for me. So I did rate it as poor although it might work as intended for flow cytometry.
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