FITC Plus Anti-Human CD71 (OKT9)

CD71 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (2)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

OKT9

Cat no : FITC-65235

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Validation Data Gallery

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  FC experiment of Human PBMC using FITC-65235

  1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9). Unactivated human PBMC were surface stained with 5ul FITC Plus Anti-human CD71 as the negative control. Cells were not fixed.

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  FC experiment of Human PBMC using FITC-65235

  1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9) or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Human PBMC

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

FITC-65235 targets CD71 in IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human,mouse	IF	Cell Chem Biol; Anti-tumor immunotherapy using engineered bacterial outer membrane vesicles fused to lysosome-targeting chimeras mediated by transferrin receptor; Authors - Ling-Yan Su