CoraLite® Plus 555 Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

H1.2F3

Cat no : CL555-65105

Synonyms

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 unstimulated or anti-CD3/CD28-stimulated mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) or 0.5 ug Isotype Control. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28-stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse dendritic epidermal T cell line Y245
• Full Name: CD69 antigen
• GenBank Accession Number: BC106997
• Gene Symbol: Cd69
• Gene ID (NCBI): 12515
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).