APC Anti-Mouse CD69 (H1.2F3)
CD69 Monoclonal Antibody for FC
Host / Isotype
Armenian Hamster / IgG
Reactivity
mouse
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
H1.2F3
Cat no : APC-65105
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Anti-CD3/CD28-treated mouse splenocytes |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 1 publications below |
Product Information
APC-65105 targets CD69 in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse |
Host / Isotype | Armenian Hamster / IgG |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mouse dendritic epidermal T cell line Y245 |
Full Name | CD69 antigen |
GenBank Accession Number | BC106997 |
Gene Symbol | Cd69 |
Gene ID (NCBI) | 12515 |
RRID | AB_2882995 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).
Protocols
Product Specific Protocols | |
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FC protocol for APC CD69 antibody APC-65105 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Sci Adv Nutrient availability regulates the secretion and function of immune cell-derived extracellular vesicles through metabolic rewiring |