CoraLite® Plus 750-conjugated CD68 Monoclonal antibody

CD68 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3A9A7

Cat no : CL750-66231

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Validation Data Gallery

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  WB analysis using CL750-66231

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66231 (CD68 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-937 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66231 targets CD68 in WB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD68 fusion protein Ag22815
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 70-100 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.